We can encapsulate cancer cell lines, primary cell lines, iPSC, iPS-derived cells (differentiated), and patient-derived cells.
Can we control it?
We have a broad range of chips to control the size of the capsule.
How do we ship our capsules?
Encapsulated tissues are sent in vials containing culture medium, on ice. This process does not affect cell viability, based on maximum 3 days of shipping time.
What is the size of the capsules?
The size of the capsule varies between 200 - 600 µm.
Are the capsules permeable?
The capsules are compatible with molecules of which molecular weight is below 100 kDa. However, we invented a new technology allowing big particles, up to the size of viruses, to cross the capsule.
3D Cell Culture
What is the size of the spheroids/organoids?
The size of the spheroid/organoids depends on the cell line, the size of the capsule, the initial number of encapsulated cells, and the age of the spheroid/organoid, however, the size is between 100-500 µm.
What is the minimal necessary number of cells required to form a spheroid/organoid?
The minimal number of cells depends on the type of cells. For cancer cell lines, we use approximately 50 cells.
Do we have necrosis?
Since the size of the spheroid/organoid can be controlled under 300 µm, we drastically limit necrosis in our encapsulated tissues.
How many cells the spheroid/organoid is made of?
After one week of encapsulation, there are on average 200 cells per spheroid/organoid.
How long can we keep encapsulated tissues (stability)?
The lifespan of the tissue depends on the type of cells. For a tissue derived from cancer cell line, up to 3 months.
How many tissues can we mix together?
The maximal number of tissues we can mix and measure is 5. Today, we can use in routine 3 tissues simultaneously.
Are the capsules selective towards some molecules?
We use alginate that is a neutral molecule (algae sugar) well described in the literature to poorly interact with most molecules. The cells grow very fast within the capsules, showing that all the nutrients and molecules that they need can easily diffuse through the capsule.