Build better drugs, together.
Whether you are a biotech with an early-stage compound, a CRO, or an academic group, FluoSphera offers partnership models adapted to your goals.
partnership models
Switzerland
FluoSphera SA, c/o FONGIT, Route de la Galaise 34
1228 Plan-les-Ouates
United States
FluoSphera Discovery Inc., 100 Cambridge Street, Boston MA
FAQ
Most common
questions.
Cell Encapsulation
01
What types of cells can we encapsulate?
We can encapsulate cancer cell lines, primary cells, iPSCs, iPSC-derived differentiated cells, and patient-derived cells.
02
What is the size of the capsules?
The standard capsule diameter is approximately 300 µm. However, depending on specific requirements, we can generate customized capsule sizes ranging from 150 to 600 µm.
03
Are the capsules permeable?
The capsules are permeable to molecules with a molecular weight below 200 kDa. We have also developed OptiCaps, a proprietary technology that enables larger particles, such as viruses, as well as cells, to reach the encapsulated spheroids.
04
What is OptiCaps?
OptiCaps is a proprietary FluoSphera technology that enables large particles and cells in suspension to reach encapsulated spheroids. It supports the study of lipid nanoparticle delivery systems, immune cell therapies, and secretome components, such as exosomes.
3D Cell Culture
01
What is the size of the spheroids or organoids?
The size of the spheroids or organoids depends on the cell type, capsule size, initial number of encapsulated cells, and culture duration. Their diameter typically ranges from 100 to 250 µm.
02
Do the tissues develop necrosis?
As the size of the spheroids or organoids can be maintained below 300 µm, necrosis within the encapsulated tissues is drastically limited.
03
What is the minimum number of cells required to form a spheroid or organoid?
The minimum number of cells depends on the cell type. For example, for cancer cell lines, we typically encapsulate approximately 20–50 cells per capsule.
04
How many cells make up a spheroid or organoid?
After one week of culture, each spheroid or organoid contains an average of approximately 200 cells.
05
How long can encapsulated tissues be maintained?
The lifespan of the tissue depends on the cell type. For example, tissues derived from cancer cell lines can be maintained for up to three weeks, while spheroids derived from immortalized cell lines can be maintained for more than three months.
Multiplexing and Tissue Communication
01
How many tissues can be combined?
We can combine up to six tissues and identify them using color coding. One example is our FluoSelect case study, in which the selectivity of 4-hydroxytamoxifen was characterized across six different cancer cell types.
02
Are the capsules compatible with immunofluorescence?
Yes. We can perform multiplexed immunofluorescence to assess five different readouts simultaneously.
Therapeutic Applications
01
Which drug modalities can be tested?
We can test a broad range of drug modalities, including small molecules such as proteolysis-targeting chimeras (PROTACs), biologics such as antibody–drug conjugates (ADCs), lipid nanoparticle delivery systems used for mRNA therapies, and cell therapy candidates.
partnership models
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